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rabbit anti ddx6 antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti ddx6 antibody
    Rabbit Anti Ddx6 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ddx6/DDX6+Antibody/us12565650-788-39-42
    Average 94 stars, based on 19 article reviews
    rabbit anti ddx6 antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: The RNA-binding protein landscapes differ between mammalian organs and cultured cells.
    Article Snippet: .. Subsequently, the Duolink PLA Fluorescence protocol (Sigma) was followed using an anti-biotin antibody (Abcam, ab201341, RRID:AB_2861249, mouse: 1:400) and either the anti-ENO1 (Proteintech, 11204-1-AP, RRID:AB_2099064, rabbit: 1:400), anti-SLC3A2 (Santa Cruz Biotechnology, sc-9160, RRID:AB_638288, rabbit: 1:400), anti-DDX6 (Novus Biologicals, NB200192, RRID:AB_10000566, rabbit: 1:400) or anti-PKM1 antibody (Cell Signaling Technology, 7067, RRID:AB_2715534, rabbit: 1:400) for detection of the protein–RNA signal. ..

    Article Title:
    Article Snippet: PRIMARY ANTIBODIES, WB: anti-ELAV-like protein 1 (ELAVL1)/Hu-antigen R (HuR) (Proteintech, 11910–1-AP, RRID:AB_11182183, 1:5000), anti-Nucleolin (Ncl) (Abcam, ab50279, RRID:AB_881762, 1:1000), anti-Beta-actin (Actb) (Sigma-Aldrich, A1978, RRID:AB_476692, 1:5000), and anti-Histone H4 (Abcam, ab10158, RRID:AB_296888, 1:4000). .. PRIMARY ANTIBODIES, PLA: anti-biotin (Abcam, ab201341, RRID:AB_2861249, mouse: 1:400), anti-ENO1 (Proteintech, 11204-1-AP, RRID:AB_2099064, rabbit: 1:400), anti-SLC3A2 (Santa Cruz Biotechnology, sc-9160, RRID:AB_638288, rabbit: 1:400), anti-DDX6 (Novus Biologicals, NB200-192, RRID:AB_10000566, rabbit: 1:400), anti-PKM1 (Cell Signaling Technology, 7067, RRID:AB_2715534, rabbit: 1:400). .. SECONDARY ANTIBODIES: anti-rabbit IgG-HRP (Abcam, ab97051, RRID:AB_10679369, 1:5000) or anti-mouse IgG-HRP (Abcam, ab6789, RRID: AB_955439, 1:5000), alpaca nanobody anti-rabbit IgG coupled with Alexa Fluor 488 (Chromotek, srbAF488-1-100, RRID:AB_2827585).

    Fluorescence:

    Article Title: The RNA-binding protein landscapes differ between mammalian organs and cultured cells.
    Article Snippet: .. Subsequently, the Duolink PLA Fluorescence protocol (Sigma) was followed using an anti-biotin antibody (Abcam, ab201341, RRID:AB_2861249, mouse: 1:400) and either the anti-ENO1 (Proteintech, 11204-1-AP, RRID:AB_2099064, rabbit: 1:400), anti-SLC3A2 (Santa Cruz Biotechnology, sc-9160, RRID:AB_638288, rabbit: 1:400), anti-DDX6 (Novus Biologicals, NB200192, RRID:AB_10000566, rabbit: 1:400) or anti-PKM1 antibody (Cell Signaling Technology, 7067, RRID:AB_2715534, rabbit: 1:400) for detection of the protein–RNA signal. ..

    Incubation:

    Article Title: Glucocorticoids Modulate mRNA Translation Fate Through P-Body Dynamics
    Article Snippet: .. Cells were grown to subconfluence and fixed at 37°C in 4% paraformaldehyde for 15 min. After fixation, cells were permeabilized with a solution containing 0.3% Triton X-100 for 5 min and incubated with blocking buffer (0.03% Triton X-100, 0.2% gelatin, and 1% BSA) for 20 min. Then, cells were incubated with primary antibodies diluted at 1:200: anti-DDX6 (Novus, distributed by Bio-Techne SAS, Noyal Châtillon sur Seiche (Fr), Cat# NB 200-191, RRID: AB_523228), anti-LSM14A (Santa Cruz Biotechnology Cat# sc-398552, RRID: AB_3099527), anti-LSM14B (Thermo Fisher Scientific Cat# PA5-66371, RRID: AB_2664653), and anti-GR (Santa Cruz Biotechnology, Cat# sc-393232, RRID: AB_2687823) overnight at 4°C in humidified chambers in blocking buffer. .. Cells were washed and incubated with Alexa Fluor-conjugated secondary antibodies at 1:500 (Thermo Fisher Scientific, Illkirch-Graffenstaden, France, anti-mouse AlexaFluor 594 (Thermo Fisher Scientific Cat# A-21203, RRID: AB_2535789) or AlexaFluor 633 (Thermo Fisher Scientific Cat# A-21050, RRID: AB_2535718), anti-rabbit AlexaFluor 488 (Cat# A-21441, RRID: AB_2535859) or AlexaFluor 594 (Cat# A-21442, RRID: AB_141840) for 1h at room temperature, washed, stained for 5 min with DAPI at 1:10,000 (Thermoscientific, Cat# 62248) and mounted with antifade fluorescence mounting medium (Abcam, Cat# AB104135).

    Blocking Assay:

    Article Title: Glucocorticoids Modulate mRNA Translation Fate Through P-Body Dynamics
    Article Snippet: .. Cells were grown to subconfluence and fixed at 37°C in 4% paraformaldehyde for 15 min. After fixation, cells were permeabilized with a solution containing 0.3% Triton X-100 for 5 min and incubated with blocking buffer (0.03% Triton X-100, 0.2% gelatin, and 1% BSA) for 20 min. Then, cells were incubated with primary antibodies diluted at 1:200: anti-DDX6 (Novus, distributed by Bio-Techne SAS, Noyal Châtillon sur Seiche (Fr), Cat# NB 200-191, RRID: AB_523228), anti-LSM14A (Santa Cruz Biotechnology Cat# sc-398552, RRID: AB_3099527), anti-LSM14B (Thermo Fisher Scientific Cat# PA5-66371, RRID: AB_2664653), and anti-GR (Santa Cruz Biotechnology, Cat# sc-393232, RRID: AB_2687823) overnight at 4°C in humidified chambers in blocking buffer. .. Cells were washed and incubated with Alexa Fluor-conjugated secondary antibodies at 1:500 (Thermo Fisher Scientific, Illkirch-Graffenstaden, France, anti-mouse AlexaFluor 594 (Thermo Fisher Scientific Cat# A-21203, RRID: AB_2535789) or AlexaFluor 633 (Thermo Fisher Scientific Cat# A-21050, RRID: AB_2535718), anti-rabbit AlexaFluor 488 (Cat# A-21441, RRID: AB_2535859) or AlexaFluor 594 (Cat# A-21442, RRID: AB_141840) for 1h at room temperature, washed, stained for 5 min with DAPI at 1:10,000 (Thermoscientific, Cat# 62248) and mounted with antifade fluorescence mounting medium (Abcam, Cat# AB104135).

    other:

    Article Title: RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules
    Article Snippet: The following primary antibodies were used in the study: human autoimmune serum containing anti-GW182 antibody were obtained as a gift from Dr. Marvin Fritzler University of Calgary; others were anti-DCP1A (cat. # ab183709, Abcam, Cambridge, MA), anti-TIA1 (clone OTI1D7, cat. # MA5-26474, Thermo Fisher Scientific), anti-G3PB1 (cat. # 611126, BD Biosciences, Franklin Lakes, NJ), anti-DDX6 (cat. # NB200-191, Novus Biologicals, Centennial, CO), anti-CNOT1 (cat. # 14276-1-AP, Proteintech, Rosemont, IL), anti-PATL1 (cat. # A303-482A, Bethyl Laboratories, Montgomery, TX), anti-4E-T/eIF4E-T (cat. # A300-706A, Bethyl Laboratories), anti-LSM14A (cat. # 18336-1-AP, Proteintech), anti-EDC3 (cat. # 16486-1-AP, Proteintech), anti-β-tubulin (cat. # T5201, Millipore Sigma), rabbit anti-FLAG (cat. # F7425, Millipore Sigma).

    Immunofluorescence:

    Article Title: Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rats
    Article Snippet: .. For immunofluorescence, the primary antibodies used were as follows: anti-AQP2 (K5007) (rabbit, 1:1000), anti-AQP2 (Santa Cruz sc-515770) (mouse 1:1000), anti–c-Myc (Cell Signaling Technologies #13987) (rabbit, 1:200), anti–c-Fos (Santa Cruz sc-166940) (mouse, 1:100), anti-ATF3 (Santa Cruz sc-81189) (mouse, 1:50), anti-DDX6 (Novus NB200–191) (rabbit, 1:500), and anti–4E-T (Abcam ab55881) (rabbit, 1:200). .. The tissue was exposed to appropriate species-specific secondary antibodies conjugated to Alexa Fluor fluorophores (Thermo Fisher Scientific).



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